anti- HIV-1 protease iBody small molecule (tool compound)
- Name Jan Tykvart ; Frantisek Sedlak ; Pavel Majer ; Petr Cigler ; Vaclav Navratil ; Vladimir Subr ; Karel Ulbich ; Jiri Strohalm ; Jan Konvalinka ; Jirí Schimer ; Tomas Knedlik ; Sacha Pavel
- Institute Institute of Organic Chemistry and Biochemistry ; Institute of Macromolecular Chemistry ; Charles University
Tool name: anti- HIV-1 protease iBody small molecule (tool compound)
Alternate names: HIV-1 PR; HIV-1 protease; HIV-2 protease; HIV1 PR; HIV2 PR; HIV1 protease
Tool type: Small molecules
Description: HIV-1 protease is a retroviral aspartyl protease (retropepsin), an enzyme involved with peptide bond hydrolysis in retroviruses, that is essential for the life-cycle of HIV, the retrovirus that causes AIDS.
- iBodiesÂŽ are capable of replacing antibodies in biomedical applications such as ELISA, flow cytometry, confocal microscopy, immunocytochemistry, Western Blot and immunoprecipitation.
- These iBodiesÂŽ consist of an N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer decorated with low-molecular-weight compounds that function as targeting ligand (e.g., enzyme inhibitors, receptor ligands), affinity anchor (e.g., biotin) and/or imaging probe (various fluorophores).
- These iBodiesÂŽ have been used for enzyme inhibition, protein isolation, immobilization, quantification and live cell imaging as well as for classical Western Blot application. The system is remarkably modular and can be used for different biological/biochemistry assays.
Research area: Cell Cycle; Cell Signaling & Signal Transduction; Immunology;
Additional notes: iBodies(R) is a registered trade mark of IOCB Tech s.r.o.
- For Research Use Only
- • Ĺ imon et al. 2018. ACS Chem Biol. 13(12):3333-3342. PMID: 30489064.
- • DvoĹĂĄkovĂĄ et al. 2017. J Med Chem. 60(20):8385-8393. PMID: 28953383.
- • Ĺ ĂĄcha et al. 2016. Angew Chem Int Ed Engl. 55(7):2356-60. PMID: 26749427.