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Contributor Information

  • Name Jan Konvalinka ; Jirí Schimer ; Tomas Knedlik ; Vaclav Navratil ; Jan Tykvart ; Frantisek Sedlak ; Pavel Majer ; Petr Cigler ; Vladimir Subr ; Karel Ulbich ; Jiri Strohalm ; Sacha Pavel
  • Institute Institute of Organic Chemistry and Biochemistry ; Institute of Macromolecular Chemistry ; Charles University

Tool Details

  • Tool name: anti-his tag iBody small molecule (tool compound)
  • Alternate names: Polyhistidine; Poly-histidine; Hexahistidine; Hexa-histidine
  • Tool type: Small molecules
  • Tool sub-type: Marker
  • Purpose: Marker
  • Description: A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. - iBodiesŽ are capable of replacing antibodies in biomedical applications such as ELISA, flow cytometry, confocal microscopy, immunocytochemistry, Western Blot and immunoprecipitation. - These iBodiesŽ consist of an N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer decorated with low-molecular-weight compounds that function as targeting ligand (e.g., enzyme inhibitors, receptor ligands), affinity anchor (e.g., biotin) and/or imaging probe (various fluorophores). - These iBodiesŽ have been used for enzyme inhibition, protein isolation, immobilization, quantification and live cell imaging as well as for classical Western Blot application. The system is remarkably modular and can be used for different biological/biochemistry assays.
  • Research area: Cell Signaling & Signal Transduction ; Other

  • For Research Use Only

Target Details

  • Target: Targeting poly-histidine (HIS) tag

Application Details

Handling

  • Purity:
  • Solubility:
  • Storage conditions: -20°C
  • Shipping conditions: Dry Ice

Documentation

  • Available on request

References

  •   Ĺ imon et al. 2018. ACS Chem Biol. 13(12):3333-3342. PMID: 30489064.
  •   DvořákovĂĄ et al. 2017. J Med Chem. 60(20):8385-8393. PMID: 28953383.
  •   Ĺ ĂĄcha et al. 2016. Angew Chem Int Ed Engl. 55(7):2356-60. PMID: 26749427.