anti-his tag iBody small molecule (tool compound)
- Name Jan Konvalinka ; Jirí Schimer ; Tomas Knedlik ; Vaclav Navratil ; Jan Tykvart ; Frantisek Sedlak ; Pavel Majer ; Petr Cigler ; Vladimir Subr ; Karel Ulbich ; Jiri Strohalm ; Sacha Pavel
- Institute Institute of Organic Chemistry and Biochemistry ; Institute of Macromolecular Chemistry ; Charles University
Tool name: anti-his tag iBody small molecule (tool compound)
Alternate names: Polyhistidine; Poly-histidine; Hexahistidine; Hexa-histidine
Tool type: Small molecules
Tool sub-type: Marker
Description: A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.
- iBodiesŽ are capable of replacing antibodies in biomedical applications such as ELISA, flow cytometry, confocal microscopy, immunocytochemistry, Western Blot and immunoprecipitation.
- These iBodiesŽ consist of an N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer decorated with low-molecular-weight compounds that function as targeting ligand (e.g., enzyme inhibitors, receptor ligands), affinity anchor (e.g., biotin) and/or imaging probe (various fluorophores).
- These iBodiesŽ have been used for enzyme inhibition, protein isolation, immobilization, quantification and live cell imaging as well as for classical Western Blot application. The system is remarkably modular and can be used for different biological/biochemistry assays.
Research area: Cell Signaling & Signal Transduction ; Other
- For Research Use Only
- • Ĺ imon et al. 2018. ACS Chem Biol. 13(12):3333-3342. PMID: 30489064.
- • DvoĹĂĄkovĂĄ et al. 2017. J Med Chem. 60(20):8385-8393. PMID: 28953383.
- • Ĺ ĂĄcha et al. 2016. Angew Chem Int Ed Engl. 55(7):2356-60. PMID: 26749427.