Penta-PT conjugate small molecule (tool compound)
- Name Czeslaw Lugowski ; Tomasz Niedziela ; Sabina Koj
- Institute Polish Center for Technology Development/Polski OĹrodek Rozwoju Technologii (PORT)
Tool name: Penta-PT conjugate small molecule (tool compound)
Tool type: Small molecules
Description: Adapted from WO2014/195881
Bordetella pertussis causes whooping cough, a highly contagious disease involving the respiratory tract, which is especially serious for infants and young children. Despite widespread immunization, in recent years the number of pertussis incidences has increased. The limited efficacy of currently used pertussis vaccines results from the fact that their components do not induce a complete immune response. Unlike tetanus and diphtheria diseases where all symptoms are caused exclusively by toxins, pertussis pathogenesis involves multiple virulence factors. Besides the secretory proteins of B. pertussis (e.g. pertussis toxin), surface molecules such as adhesins and endotoxin are involved in pathogenesis of pertussis. Therefore the pertussis vaccine needs to provide a functional immunity in the form of the toxin neutralization, blocking of bacterial adherence, opsonization, complement activation and bacterial killing. There is a need for a pertussis vaccine containing immunogenic, but completely inactivated components of B. pertussis.
Research area: Drug Discovery & Development; Immunology
Selectivity: This pentasaccharide-PT glycoconjugate is not capable of binding to fetuin in ELISA test.
Additional notes: Adapted from WO2014/195881
Glycoconjugate of the distal pentasaccharide obtained by deamination of LOS (from Bordetella pertussis) conjugated to a pertussis toxin. The pentasaccharide part of the conjugate is a fragment isolated from the LOS of B. pertussis 186 and it comprises a distal trisaccharide, a heptose and an anhydromannose. It has been shown that pentasaccharide-TTd conjugate induced antibodies which were able to bind to B. pertussis in immunofluorescence assays (FACS). Moreover, using STD-NMR techniques it was confirmed that that epitopes which are involved in antigen-antibody recognition are located in the distal trisaccharide and the heptose.
- For Research Use Only
- • WO2014195881 - BORDETELLA PERTUSSIS LOS-DERIVED OLIGOSACCHARIDE WITH PERTUSSIS TOXIN GLYCOCONJUGATE AND ITS APPLICATION IN THE PROPHYLAXIS AND TREATMENT OF INFECTIONS CAUSED BY BORDETELLA PERTUSSIS