4-trifluoromethyl-7-hydrazinyl-2H-chromen-2-one (TFCH) biomolecular fluorophore small molecule (tool compound)
- Name Susan Bane ; Kamalika Mukherjee
- Institute Binghamton University
Tool name: 4-trifluoromethyl-7-hydrazinyl-2H-chromen-2-one (TFCH) biomolecular fluorophore small molecule (tool compound)
Alternate names: TFCH
Tool type: Small molecules
Tool sub-type: Fluorescent Probe
Application: Renal tissue imaging. Live cell screening
Description: 4-trifluoromethyl-7-hydrazinyl-2H-chromen-2-one (TFCH) is a novel fluorescent biocompatible probe has been developed to detect oxidative damage in live cells and tissues in as little as 5 minutes. TO accomplish this, TFCH utilizes the irreversible reaction of radicals with biomolecules that result in the covalent addition of carbonyls to biomolecules. This probe has been shown to outperform other commercially available toxicity and reactive oxygen species assays. This probe has been shown to be highly sensitive. The limit of detection is orders of magnitude smaller than other previously reported fluorophores resulting in less reagent needed to detect carbonylation.TFCH is rapid, live cell and tissue compatible and also be used in fixed cells and tissues.
Research area: Drug Discovery & Development; Fluorescent Cell Imaging
- For Research Use Only
- • A Highly Sensitive Fluorogenic Assay for the Detection of Nephrotoxin-Induced Oxidative Stress in Live Cells and Renal Tissue
- • Mukherjee et al. 2021. ACS Sens. 6(7):2523-2528. PMID: 34214393.