- Name Jesper Svejstrup
- Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool name: MultiDsk Vector
Tool type: Vector
Description: Ubiquitylation is a highly diverse and complex post-translational modification for the regulation of protein function and stability. Studies of ubiquitylation have, however, been hampered by its rapid reversal in cell extracts, for example through the action of de-ubiquitylating enzymes (DUBs). MultiDsk is a novel ubiquitin-binding protein reagent and is composed of an array of five UBA domains from the yeast ubiquitin-binding protein Dsk2, fused to GST. MultiDsk binds ubiquitylated substrates with unprecedented avidity, and can be used as both an affinity resin to study protein ubiquitylation, and to effectively protect ubiquitylated proteins from the action of DUBs and the proteasome in crude cell extracts. MultiDsk offers the opportunity to obtain pure, native, nontagged, ubiquitylated proteins from cell extracts whilst helping ensure the maintenance of the normal ubiquitylation state via a strong protective function, inhibiting de-ubiquitylation.
Research area: Cell signaling and signal transduction; Genetics
- For Research Use Only
- • Emmerich et al. 2015. Biochem Biophys Res Commun. :. PMID: 26325464.
- • Optimising methods for the preservation, capture and identification of ubiquitin chains and ubiquitylated proteins by immunoblotting.
- • Wilson et al. 2013. Cell. 154(5):983-95. PMID: 23993092.
- • Proteasome-mediated processing of Def1, a critical step in the cellular response to transcription stress.
- • MultiDsk: a ubiquitin-specific affinity resin.
- • Wilson et al. 2012. PLoS One. 7(10):e46398. PMID: 23056298.