pJEK4 Alpha-Synuclein A30P Vector
- Name Dr Fiona Benson
- Institute Lancaster University
Tool name: pJEK4 Alpha-Synuclein A30P Vector
Tool type: Vector
Description: pJEK4 ("A30P") is a derivative of pET15b with the open reading frame encoding the A30P mutant human a-synuclein cloned in via the NdeI and BamHI restriction sites. It was constructed via site-specific mutagenesis of pJEK1, replacing the G at position 88 in the ORF nucleotide sequence with a C, thus altering the 30th codon from GCA encoding Ala, to CCA encoding Pro. In this construct A30P alpha-synuclein is expressed as a fusion protein with an N-terminal six His tag.
Research area: Neurobiology
- For Research Use Only