VECTORS
Contributor Information
- Name Dr Fiona Benson
- Institute Lancaster University
Tool Details
- Tool name: pJEK5 Alpha-Synuclein A53T Vector
- Tool type: Vector
- Description: pJEK5 ("A53T") is a derivative of pET15b with the open reading frame encoding the A53T mutant human ĂÄ -synuclein cloned in via the NdeI and BamHI restriction sites. It was constructed via site-specific mutagenesis of pJEK1, replacing the G at position 88 in the ORF nucleotide sequence with a C, thus altering the 30th codon from GCA encoding Ala, to CCA encoding Pro. In this construct A30P alpha-synuclein is expressed as a fusion protein with an N-terminal six His tag.
- Research area: Neurobiology
- For Research Use Only
Target Details
Application Details
Handling
Documentation
- Available on request
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