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Contributor Information

  • Name Dr Fiona Benson
  • Institute Lancaster University

Tool Details

  • Tool name: pJEK6 Alpha-Synuclein E46K Vector
  • Tool type: Vector
  • Description: pJEK6 ("E46K") is a derivative of pET15b with the open reading frame encoding the E46K mutant human alpha synuclein (Îą-synuclein) cloned in via the NdeI and BamHI restriction sites. It was constructed via site specific mutagenesis of pJEK1, replacing the G at position 136 in the ORF nucleotide sequence with A, thus altering the 46th codon from GAG encoding Glutamic Acid (E) to AAG encoding Lys (K). In this construct E46K Îą-synuclein is expressed as a fusion protein with an N-terminal six His tag.
  • Research area: Neurobiology

  • For Research Use Only

Target Details

Application Details