pKH1 Beta-Synuclein V70M Vector
- Name Dr Fiona Benson
- Institute Lancaster University
Tool name: pKH1 Beta-Synuclein V70M Vector
Tool type: Vector
Description: pKH1 ("V70M") is a derivative of pET15b with the open reading frame encoding the V70M mutant human beta-synuclein (ÄÂĂÂ-synuclein) cloned in via the vector NdeI and BamHI restriction sites. It was constructed via site-specific mutagenesis of pJEK12 (pET15b-wt ÄÂĂÂ-synuclein), replacing the G at position 208 in the ORF nucleotide sequence with A, thus altering the 70th codon from GTG encoding valine (V) to ATG encoding methionine (M). In this construct V70M ÄÂĂÂ-synuclein is expressed as a fusion protein with an N-terminal six His tag.
Research area: Neurobiology
- For Research Use Only