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Contributor Information

  • Name Dr Fiona Benson
  • Institute Lancaster University

Tool Details

  • Tool name: pKH1 Beta-Synuclein V70M Vector
  • Tool type: Vector
  • Description: pKH1 ("V70M") is a derivative of pET15b with the open reading frame encoding the V70M mutant human beta-synuclein (β-synuclein) cloned in via the vector NdeI and BamHI restriction sites. It was constructed via site-specific mutagenesis of pJEK12 (pET15b-wt β-synuclein), replacing the G at position 208 in the ORF nucleotide sequence with A, thus altering the 70th codon from GTG encoding valine (V) to ATG encoding methionine (M). In this construct V70M β-synuclein is expressed as a fusion protein with an N-terminal six His tag.
  • Research area: Neurobiology

  • For Research Use Only

Target Details

Application Details

Handling

Documentation

  • Available on request