pKH2 Beta-Synuclein P123H Vector
- Name Dr Fiona Benson
- Institute Lancaster University
Tool name: pKH2 Beta-Synuclein P123H Vector
Tool type: Vector
Description: pKH2 ("P123H") is a derivative of pET15b with the open reading frame encoding the P123H mutant human beta-synuclein (Î˛-synuclein) cloned in via the vector NdeI and BamHI restriction sites. It was constructed via site-specific mutagenesis of pJEK12 (pET15b-wt Î˛-synuclein), replacing the C at position 368 in the ORF nucleotide sequence with A, thus altering the 123rd codon from CCC encoding proline (P) to CAC encoding histidine (H). In this construct P123H Î˛-synuclein is expressed as a fusion protein with an N-terminal six His tag.
Research area: Neurobiology
- For Research Use Only