- Name Helen McCarthy
- Institute Queen's University Belfast
Tool name: phOC-iNOS Vector
Alternate names: Nitric Oxide Synthase
Tool type: Vector
Tool sub-type: pcDNA 3.1
Description: phOC-iNOS Plasmid is a human nitric oxide synthase (iNOS) transgene driven by a human osteocalcin (hOC) promoter to control inducible nitric oxide synthase (iNOS) transgene expression in hormone refractory prostate cancer (HRPC). The plasmid was originally developed as an anti-cancer therapeutic with a view to a gene therapy strategies. Studies in the literature have demonstrated that this plasmid increases iNOS protein and total nitrite levels in PC3 and DU145 cells, but not LNCaP or
HT29 (McCarthy et al., 2007). The plasmid serves as a useful tool in cardiovascular studies or any physiology associated with vasodilation.
Research area: Cancer; Metabolism; Neurobiology
Bacterial resistance: Kanamycin
- For Research Use Only
- • McCarthy et al. 2007. J Gene Med. 9(6):511-20. PMID: 17471586.
- • Human osteocalcin: a strong promoter for nitric oxide synthase gene therapy, with specificity for hormone refractory prostate cancer.