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Contributor Information

  • Name Dr Andrew Stephens ; Amy Wilson
  • Institute Hudson Institute Of Medical Research

Tool Details

  • Tool name: pROSA-GUIDE
  • Tool type: Vector
  • Tool sub-type: Mammalian Expression, Lentiviral, CRISPR
  • Description: Validated gRNA targeting ROSA26 locus in mouse cells. Use in combination with pROSA-puro-GFP CRISPR or pROSA-puro-iRFP720 CRISPR to integrate any gene of interest at ROSA26 locus in mouse cells. gRNA in plentiCRISPRv1 backbone. gRNA sequence based on Quadros et al 2015 PMID: 25834785. Species: Mus musculus (house mouse) Cloning method: Restriction Enzyme 5’ cloning site: BsmbI 3’ cloning site: BsmbI 5’ sequencing primer: U6 Forward
  • Research area: Fluorescent Cell Imaging ; Gene Expression
  • Bacterial resistance: Ampicillin
  • Selectable markers: Puromycin

  • For Research Use Only

Target Details

  • Target: Gt(ROSA)26Sor; ROSA26

Application Details

Handling

Documentation

  • Available on request