VECTORS
Contributor Information
- Name Dr Andrew Stephens ; Amy Wilson
- Institute Hudson Institute Of Medical Research
Tool Details
- Tool name: pROSA-GUIDE
- Tool type: Vector
- Tool sub-type: Mammalian Expression, Lentiviral, CRISPR
- Description: Validated gRNA targeting ROSA26 locus in mouse cells. Use in combination with pROSA-puro-GFP CRISPR or pROSA-puro-iRFP720 CRISPR to integrate any gene of interest at ROSA26 locus in mouse cells. gRNA in plentiCRISPRv1 backbone. gRNA sequence based on Quadros et al 2015 PMID: 25834785. Species: Mus musculus (house mouse) Cloning method: Restriction Enzyme 5ÄËĂÂĂÂ cloning site: BsmbI 3ÄËĂÂĂÂ cloning site: BsmbI 5ÄËĂÂĂÂ sequencing primer: U6 Forward
- Research area: Cell biology; Genetics
- Bacterial resistance: Ampicillin
- Selectable markers: Puromycin
- For Research Use Only
Target Details
- Target: Gt(ROSA)26Sor; ROSA26
Application Details
Handling
Documentation
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