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Contributor Information

  • Name Dr. Patrick Loll
  • Institute Drexel University

Tool Details

  • Tool name: dtUD1 vector
  • Tool type: Vector
  • Tool sub-type: pETSU vector containing an untagged yeast SUMO sequence
  • Description: Affinity-tagged small ubiquitin related modifier (SUMO) fusion proteins generated by the cloning vectors: pETHSUL, pETS2SUL, pASHSUL, or pASS2SUL are treated with recombinant SUMO protease, generated from the pdtUD1 vector, to cleave SUMO and yield the mature target protein. Instructions for vector expression, purification, and use are outlined in: Weeks, S. D., Drinker, M., & Loll, P. J. (2007). Ligation independent cloning vectors for expression of SUMO fusions. Protein expression and purification, 53(1), 40–50. doi:10.1016/j.pep.2006.12.006 Protein Purification; Protein Biochemistry
  • Research area: Other
  • Bacterial resistance: Ampicillin

  • For Research Use Only

Target Details

  • Target: A dual tagged clone of the catalytic domain (dtUD1) of the S. cerevisiae SUMO hydrolase. The recombinant protease has an amino terminal Strep-II tag and a carboxy terminal His6 tag.

Application Details

Handling

Documentation

  • Available on request

References

  •   Weeks et al. 2007. Protein Expr Purif. 53(1):40-50. PMID: 17251035.