- Name Dr. Patrick Loll
- Institute Drexel University
Tool name: dtUD1 vector
Tool type: Vector
Tool sub-type: pETSU vector containing an untagged yeast SUMO sequence
Description: Affinity-tagged small ubiquitin related modifier (SUMO) fusion proteins generated by the cloning vectors: pETHSUL, pETS2SUL, pASHSUL, or pASS2SUL are treated with recombinant SUMO protease, generated from the pdtUD1 vector, to cleave SUMO and yield the mature target protein.
Instructions for vector expression, purification, and use are outlined in: Weeks, S. D., Drinker, M., & Loll, P. J. (2007). Ligation independent cloning vectors for expression of SUMO fusions. Protein expression and purification, 53(1), 40ÄËĂÂĂÂ50. doi:10.1016/j.pep.2006.12.006
Protein Purification; Protein Biochemistry
Research area: Other
Bacterial resistance: Ampicillin
- For Research Use Only
- • Weeks et al. 2007. Protein Expr Purif. 53(1):40-50. PMID: 17251035.