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Contributor Information

  • Name LuĂ­sa V Lopes
  • Institute Instituto de Medicina Molecular JoĂŁo Lobo Antunes

Tool Details

  • Tool name: Venus-A2AR vector
  • Alternate names: A2AR
  • Tool type: Vector
  • Tool sub-type: pcDNA3.1( + )
  • Description: Venus-A2AR construct was generated with the In-fusion HD Cloning Kit (Clontech Takara, USA). Venus and A2AR fragments were produced by using, respectively, the pair of primers 5'-GTTTAAACTTAAGCTTATGGTGAGCAAGGGCGAG-3' and 5'-GCTGCCCATGGTGGCCTTGTACAGCTCGTCCATG-3', and the pair of primers 5'-GCCACCATGGGCAGCAGC-3' and 5'-AAACGGGCCCTCTAGATCAGCTGGGGGCGAACTC-3'. PCR fragments were cloned into the vector pcDNA3.1( + ) linearized with HindIII and XbaI, and the resulting construct was verified by DNA sequencing (GATC Biotech, Germany).
  • Research area: Fluorescent Cell Imaging ; Neurobiology
  • Bacterial resistance: Ampicillin
  • Selectable markers: Neomycin

  • For Research Use Only

Target Details

  • Target: Adenosine A2A receptor

Application Details

Handling

Documentation

  • Available on request

References

  •   Temido-Ferreira et al. 2020. Mol Psychiatry. 25(8):1876-1900. PMID: 29950682.