- Name LuĂÂĂÂsa V Lopes
- Institute Instituto de Medicina Molecular JoĂÂÄšÂo Lobo Antunes
Tool name: Venus-A2AR vector
Alternate names: A2AR
Tool type: Vector
Tool sub-type: pcDNA3.1(ÄËĂÂĂÂ+ÄËĂÂĂÂ)
Description: Venus-A2AR construct was generated with the In-fusion HD Cloning Kit (Clontech Takara, USA). Venus and A2AR fragments were produced by using, respectively, the pair of primers 5'-GTTTAAACTTAAGCTTATGGTGAGCAAGGGCGAG-3' and 5'-GCTGCCCATGGTGGCCTTGTACAGCTCGTCCATG-3', and the pair of primers 5'-GCCACCATGGGCAGCAGC-3' and 5'-AAACGGGCCCTCTAGATCAGCTGGGGGCGAACTC-3'. PCR fragments were cloned into the vector pcDNA3.1(ÄËĂÂĂÂ+ÄËĂÂĂÂ) linearized with HindIII and XbaI, and the resulting construct was verified by DNA sequencing (GATC Biotech, Germany).
Research area: Cell biology; Neurobiology
Bacterial resistance: Ampicillin
Selectable markers: Neomycin
- For Research Use Only
- • Temido-Ferreira et al. 2020. Mol Psychiatry. 25(8):1876-1900. PMID: 29950682.