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Contributor Information

  • Name Tina Sket ; Andrii Domanskyi
  • Institute University Of Helsinki

Tool Details

  • Tool name: pTO-sp-XBP1-NLuc-FRT XBP1 splicing reporter plasmid
  • Alternate names: XBP1-NLuc reporter
  • Tool type: Vector
  • Tool sub-type: pTO-HA-Strep-GW-FRT backbone for creation of stable cell line using Flp-In HEK-293 T-Rex cells
  • Description: secNLuc-XBP1 fragment (876 bp) can be subcloned by using HindIII and ApaI restriction sites The plasmid can be used in transient transfections to cells, which do not express Tet repressor protein
  • Research area: Cancer ; Neurobiology
  • Bacterial resistance: Ampicillin
  • Selectable markers: Hygromycin

  • For Research Use Only

Target Details

  • Target: activation of IRE1 branch of unfolded protein response

Application Details

Handling

Documentation

  • Available on request