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Contributor Information

  • Name Stefan Schild
  • Institute University of Graz

Tool Details

  • Tool name: TetR-controlled recombination-based in vivo expression technology (TRIVET) vector
  • Alternate names: TRIVET
  • Tool type: Vector
  • Tool sub-type: Reporter
  • Description: This system is based upon recombination-based in vivo expression technology (RIVET) and allows the detection of transient gene silencing events even in a subset of a complex, heterogenous bacterial population. It requires all three components; the tpc reporter cassette, pTRIVET and pTRIVET1. It can be used as a random approach to identify in vivo repressed genes during intestinal colonization and as a specific approach to study conditional transcriptional silencing of a gene of interest. The protocol for which can be found at PMID: 33659431.
  • Research area: Microbiology
  • Bacterial resistance: pTRIVET - ampicillin, tpc reporter cassette - chloramphenicol
  • Selectable markers: PhoA activity

  • For Research Use Only

Target Details

  • Target: Cholera

Application Details

Handling

Documentation

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References

  • •  33659431
  • •  Cakar et al. 2018. Proc Natl Acad Sci U S A. 115(10):E2376-E2385. PMID: 29463743.