TetR-controlled recombination-based in vivo expression technology (TRIVET) vector
- Name Stefan Schild
- Institute University of Graz
Tool name: TetR-controlled recombination-based in vivo expression technology (TRIVET) vector
Alternate names: TRIVET
Tool type: Vector
Tool sub-type: Reporter
Description: This system is based upon recombination-based in vivo expression technology (RIVET) and allows the detection of transient gene silencing events even in a subset of a complex, heterogenous bacterial population. It requires all three components; the tpc reporter cassette, pTRIVET and pTRIVET1. It can be used as a random approach to identifyÄËĂÂÄšĹĽin vivoÄËĂÂÄšĹĽrepressed genes during intestinal colonization and as a specific approach to study conditional transcriptional silencing of a gene of interest. The protocol for which can be found at PMID: 33659431.
Research area: Microbiology
Bacterial resistance: pTRIVET - ampicillin, tpc reporter cassette - chloramphenicol
Selectable markers: PhoA activity
- For Research Use Only
- • 33659431
- • Cakar et al. 2018. Proc Natl Acad Sci U S A. 115(10):E2376-E2385. PMID: 29463743.