VECTORS
Contributor Information
- Name Gontzal GarcĂa del CaĂąo
- Institute University of the Basque Country
- Primary citation Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.
Tool Details
- Tool name: IPTG-inducible pGEX-6P1 expression vector for GST and human CB1 receptor (AA 414 to 472) fusion protein
- Tool type: Vector
- Tool sub-type: IPTG-inducible pGEX-6P1 expression vector
- Sequence: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.
- Insert: GST-CB1414â472
- Application: GST fusion protein production
- Cloning information: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.
- Description: Purified plasmid DNA containing the IPTG inducible coding sequence for the polypeptide spanning amino acids 414 to 472 of the cytosolic tail of the human CB1 receptor fused to the C-terminus of the glutathione S-transferase tag (GST-CB1414â472)
- Research area: Neurobiology; Cell biology; Molecular Biology; Biochemistry
- Tag: glutathione S-transferase (GST)
- Total vector size: 5138 bp
- Backbone size without insert: 4984 bp
- Insert size: 183 bp
- Insert species: Human
- Promoter: Isopropyl ?-D-thiogalactoside (IPTG)-inducible tac promoter. The vector is designed with an internal lacIq gene whose protein product binds to the operator region of the tac promoter. Thus, expression is repressed unless IPTG is added to the culture, thus maintaining tight control over the expression of the insert.
- Bacterial resistance: Ampicillin
- Additional notes: Contact the inventor for further information.
- For Research Use Only
Target Details
Application Details
- Application: GST fusion protein production
Handling
- Storage buffer: Double distilled water
- Storage conditions: Refrigerated at 4 oC (or frozen at -20 oC for long periods)
- Shipping conditions: At room temperature in water solution
Documentation
Related Tools
References
- • GĂłmez-Caballero et al. (2021). Mikrochim Acta, 188:368. doi:10.1007/s00604-021-05029-z
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