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Contributor Information

  • Name Gontzal GarcĂ­a del CaĂąo
  • Institute University of the Basque Country
  • Primary citation Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.

Tool Details

  • Tool name: pCR-Blunt II-TOPO™ cloning vector for GST and human CB1 receptor (AA 414 to 472) fusion protein
  • Tool type: Vector
  • Tool sub-type: pCR-Blunt II-TOPO™ cloning vector
  • Sequence: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.
  • Insert: CB1414–472
  • Application: Subcloning
  • Cloning information: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.
  • Description: Purified plasmid with a DNA insert spanning codons corresponding to amino acids CB1414-472 of the cytosolic tail of the human CB1 receptor (CB1414–472) with BamHI and NotI restriction sites for subcloning
  • Research area: Neurobiology; Cell biology; Molecular Biology; Biochemistry
  • Total vector size: 3710 bp
  • Backbone size without insert: 3519 bp
  • Insert size: 191 bp
  • Insert species: Human
  • Promoter: Not applicable
  • Bacterial resistance: Kanamycin
  • Additional notes: Contact the inventor for further information.

  • For Research Use Only

Target Details

Application Details

  • Application: Subcloning

Handling

  • Storage buffer: Double distilled water
  • Storage conditions: Refrigerated at 4 oC (or frozen at -20 oC for long periods)
  • Shipping conditions: At room temperature in water solution

Documentation

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References

  • •  GĂłmez-Caballero et al. (2021). Mikrochim Acta, 188:368. doi:10.1007/s00604-021-05029-z